GENOMICS

Kasp400.gifSNP analysis by real-time PCR, using Kasp primers on a RT-PCR machine in the lab.




CEQTrace400.gifTrace of multiplexed SSR markers using an automated DNA sequencer in the laboratory.









Goals:

 

          Use genetic markers for marker-assisted breeding to develop better peanut cultivars.

 

          Identify new markers by QTL and association mapping.

 

          Develop improved genetic maps of peanut.

 

          Collaborate with the International Peanut Genome Initiative in developing the genome and transcriptome sequences of peanut.


References

Burow M. D., J. L. Starr , C.-H. Park , C. E. Simpson, A. H. Paterson. (2014) Introgression of Homoeologous QTLs for Resistance to the Root-knot Nematode (Meloidogyne arenaria (Neal) Chitwood) in an Advanced Backcross-QTL Population of Peanut (Arachis hypogaea L.) Mol Breeding. 10.1007/s11032-014-0042-2

 

Holbrook, C. C., T.G. Isleib , P. Ozias-Akins , Y. Chu , S.J. Knapp , B. Tillman , B. Guo , R. Gill, and M. D. Burow. (2013) Development and Phenotyping of Recombinant Inbred Line (RIL) Populations for Peanut (Arachis hypogaea). Peanut Science 40:89–94.

 

Belamkar, V., M. G. Selvaraj, J. L. Ayers, P. Payton, N. Puppala, and M. D. Burow. (2011) A first insight into population structure and linkage disequilibrium in the US peanut minicore collection. Genetica 139:411-29.

 

Kottapalli, P., P. Payton, M. D. Burow, H. Upadhyaya, S. Dwivedi, S. Sanogo, and N. Puppala. (2011) Population Structure and Diversity in Valencia Peanut Germplasm Collection. Crop Science 51: 1089-1100.

 

Cason, J. M., C. E. Simpson, J. L. Starr, and M. D. Burow. (2010) Marker-assisted selection in the transfer of root-knot nematode resistance in the commercial peanut (Arachis hypogaea L.). Texas J. Science 62(1).

 

Burow, M. D., C. E. Simpson, M. W. Faries, J. L. Starr, and A. H. Paterson. (2009) Molecular biogeographic study of recently-described B-genome Arachis species, also providing new insights into the origins of cultivated peanut. Genome 52: 107-119.

 

Payton, P., K. R. Kottapalli, D. Rowland, W. Faircloth, B. Guo, M. Burow, N. Puppala, and M. Gallo. (2009) Gene expression profiling in peanut using high density oligonucleotide microarrays. BMC Genomics 10:265, pp. 1-11.

 

Gomez, S. M, N. N. Denwar, T. Ramasubramanian, C. E Simpson, G Burow, J.J Burke, N Puppala, and M.D Burow. (2008) Identification of Peanut Hybrids Using Microsatellite Markers and Horizontal Polyacrylamide Gel Electrophoresis. Peanut Sci 35: 123–129.

 

Kottapalli, K. R., M. D. Burow, G. Burow, J. Burke, and N. Puppala. (2007) Characterization of the Core of the U. S. Peanut Core Germplasm Collection using SSR Markers. Crop Sci. 47: 1718-1727.

 

Jesubatham, A. M., and M. D. Burow. (2006) PeanutMap: An Online Genome Database for Comparative Molecular Maps of Peanut. BMC Bioinformatics 7: 375.

 

Ferguson, M. E., M. D. Burow, S. R. Schulze, P. J. Bramel, A. H. Paterson, S. Kresovich, and S. Mitchell. (2004) Microsatellite identification and characterization in peanut. (A. hypogaea L.) Theor. Appl. Genet. 108: 1064-1070.

 

Krishna, G., J. Zhang, M. Burow, R. N. Pittman, S. G. Delikostadinov, and N. Puppala. (2004) Genetic Diversity Analysis in Valencia Peanut (Arachis hypogaea L.) using Microsatellite Markers. Cell. Mol. Biol. Lett. 9: 685-697.

 

Yang, M., R. Bower, M. D. Burow, A. H. Paterson, and T. E. Mirkov. (2003) A Rapid and Direct Approach to Identify Promoters That Confer High Levels of Gene Expression in Monocots. Crop Sci. 43: 1805-1814.

 

Burow, M. D., C. E. Simpson, J. L. Starr, and A. H. Paterson. (2001) Transmission genetics of chromatin from a synthetic amphidiploid to cultivated peanut (Arachis hypogaea L.): broadening the gene pool of a monophyletic polyploid species. Genetics 159: 823-837.

 

Nguyen, V. T., M. D. Burow, H. T. Nguyen, B. T. Le, T. D. Le, and A. H. Paterson. (2001) Molecular mapping of genes conferring aluminum tolerance in rice (Oryza sativa L.) Theor. Appl. Genet. 102: 1002-1010.

 

Paterson, A. H., J. E. Bowers, M. D. Burow, X. Draye, C. Elsik, C.-X. Jiang, C. S. Katsar, T.-H. Lan, Y.-R. Lin, R. Ming, and R. J. Wright. (2000). Comparative organization and function of plant chromosomes. Plant Cell 12: 1523-1539.

 

Church, G. T., C. E. Simpson, M. D. Burow, A. H. Paterson, and J. L. Starr. (2000) RFLP markers for identification of resistance genotype in peanut. Nematology 2: 575-580.

 

Wang, X., M. Burow, P. Felker, and A. Paterson. (1998). Evaluating genetic relationships of Opuntia spp. using randomly-amplified polymorphic DNA (RAPDs). J. Prof. Assoc. Cactus Devel. 3: 3-14.

 

Burow, M. D., C. E. Simpson, A. H. Paterson, and J. L. Starr. (1996) Identification of peanut (Arachis hypogaea) RAPD markers diagnostic of root-knot nematode (Meloidogyne arenaria (Neal) Chitwood) resistance. Mol. Breeding 2: 307-319.

 

Paterson, A. H., D. L. Lan, K. P. Reischmann, J. L. Chang, Y. R. Lin, S. C. Liu, M. D. Burow, S. P. Kowalski, C. S. Katsar, T. A. DelMonte, K. A. Feldmann, K. F. Schertz, and J. F. Wendel. (1996) Toward a unified map of higher plant chromosomes, transcending the monocot-dicot divergence. Nature Genet. 14: 380-382.

 

Sen, P., C. A. Chlan, M. D. Burow, W. S. Lee, and N. Murai. (1993) Apical and lateral shoot apex-specific expression is conferred by a promoter of the seed storage protein ß-phaseolin gene. Transgenic Research 2: 21-28.

 

Burow, M. D., P. Sen, C. A. Chlan, and N. Murai. (1992) Developmental control of the ß-phaseolin gene requires positive, negative, and temporal seed-specific transcriptional regulatory elements and a negative element for stem and root expression. Plant J. 2: 537-548.

 

Burow, M. D., C. A. Chlan, P. Sen, A. Lisca, and N. Murai. (1990) High-frequency generation of tobacco plants after modified leaf disk cocultivation with Agrobacterium tumefaciens. Plant Mol. Biol. Rep. 8: 124-139.

 

Li, Z., M. D. Burow, and N. Murai. (1990) High-frequency generation of fertile transgenic rice plants after PEG-mediated protoplast transformation. Plant Mol. Biol. Rep. 8: 276-291.